1. During the office visit, a small amount of a patient's own blood is drawn into a vacuum collection tube containing a cell separator gel.

2. The tube is then placed into a centrifuge and spun for six minutes to separate the blood into a supernatant plasma/platelet suspension.

3. The red and white blood cells are located below the cell separator gel.

4. The platelets and plasma are then transferred in a closed system to a second vacuum tube containing a small amount of calcium chloride solution.

5. Fibrin polymerization begins and the PRP remains liquid for approximately 10 minutes before forming

PREM.
6. The patient's own PREM is ready for use as deemed appropriate by the clinical use requirements.